|
KMID : 0351619720130020157
|
|
Kyungpook Medical Journal
1972 Volume.13 No. 2 p.157 ~ p.169
|
|
|
Electron Microscopic Observation of Reversibility of Ethionine Induced Liver Cell Injury by Adenine
|
|
|
|
|
Abstract
|
|
|
There are many reports about the hepatotosicity of thionine and the reversibilit}r of the injury by methionine, adenine, inosine; and so on. Ethionine induces degenerative changes of the liver cell nucleus and various cytoplasmic organells for which adenine may reverse and proven*. such alterations of them. The purpose of this observation is to review the alterations of c toplasmic organelle and nucleus and to atte~ip to study the relationship between structural and biochemical changes of hepatic cells, especially of those structures related to the lysosomes.
DL-Ethionine (1 mg/ gm of body weight) vas injected intraperitoneally to 24 rats and adenine sulfate (32mg) was rsinjected intraperitoneaily to the half of them 6hrs after initial injection. Control groups were received saline only, e~hionine and saline, or saline and aden ine respectively. Each group of animals vas killed at 3, 6, 9 and 12 ham´ after -the lastinjection respectively and the livers were taken. from the rats. Sections of the tissue ,vere observed under the light and election microscopee:
The liver cells showed slight cloudy swelling in early time and progresssively severer in later time, but milder in adenine treated group. On the electron microscopical levels, liver cells showed dilatation of endoplasmic reticulum, shedding of ribosome, swelling and partial disruption of mitochondria, and increase of various types of the lysosomes. Most of the lysosomes in early stage were primary type but changed to secondary type in later time. Adenine in jection induced production of new primary lysosome and recovering of the alterations of cytoplasmic organells. The results suggest that adenine can reverse ethionine induced alterations of the organells but already injured organells go on destruction by newly formed lysosomes.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|